From 2007.igem.org

Contents 1 Cis-acting Bio-Logic Gates 2 Model 3 Schemes of Bio-Logic Promoters 4 Repression Assay 4.1 Build Up Promoter Family 4.2 Solo-Repression Assay 4.3 Co-Repression Assay 5 Final Results 6 References

Cis-acting Bio-Logic Gates

Model

Schemes of Bio-Logic Promoters

Repression Assay

Build Up Promoter Family

Firstly, we extend both sides of the conservative region for transcriptional initiation of PlacUV5[], including -35 box，-10 box and +1 starting point, with two non-sense sequence selected from random groups. The product is named as P_template1 as it is the template for the promoter family. These two non-sense sequence have three main characters:

1. They will never include the restriction enzyme cutting sites that will be involved in the whole study;
2. They will never include the recognition sites of RNA Polymerases and those of either of the two repressors;
3. They will never present in complicated structures.

Secondly, another group of primers, of which the elongation region at 5’ end may contain a unique operator sequence or each, is applied at both ends of P_template1, equipping us with an according group of promoters with complete structures. These promoters can include variant operator sequences at different position in flank of the conservative region.

Solo-Repression Assay

Co-Repression Assay

Final Results

References