Tokyo/Assay
From 2007.igem.org
Contents |
Hill function fitting
新しく作ったハイブリッドプロモーターに関してはデータがないので,実験によりパラメータを求める.
ヒル関数をfittingさせることにより,n2,n3,K2,K3が求まる.
We have obtained data for our newly devised hybrid promoter. By Hill function fitting, we have determined n2, n3, K2, and K3.
導出に関してはfor more detail.
Preliminary assays
Preliminaly assays were done
- to practice and experience the assays with AHL and IPTG
- to check if and how our materials actually worked.
AHL preparation
Experimental precision and accurecy
AHL assay practice
Lux-lac hybrid promoter activation check
OD correction
Wash
New AHL check
Tokyo/Hybrid promoter activation & repression check
Assays for hybrid promoter
These assays were done
-to determine the characteristics of our hybrid promoter.
The data from here were used for computational analyses and simulations.