From 2007.igem.org

10/08/07

• We inoculate:

-[http://partsregistry.org/Part:BBa_I763031 I763031] (low copy plasmid);

-[http://partsregistry.org/Part:BBa_I763016 I763016] (low copy plasmid);

-E.Coli;

-[http://partsregistry.org/Part:BBa_I763032 I763032] (high copy plasmid).

• Digestion of:

-[http://partsregistry.org/Part:BBa_I763035 I763035] with Eco/Pst1;

-[http://partsregistry.org/Part:BBa_I763007 I763007] with Eco/spe;

-[http://partsregistry.org/Part:BBa_I763026 I763026] with Eco/Xba;

-[http://partsregistry.org/Part:pSB4A3 pSB4A3] with Spe/Xba.

• Ligation for:

-[http://partsregistry.org/Part:BBa_I763035 I763035]+[http://partsregistry.org/Part:pSB4A3 pSB4A3];

-[http://partsregistry.org/Part:BBa_I763007 I763007]+[http://partsregistry.org/Part:BBa_I763026 I763026];

-[http://partsregistry.org/Part:BBa_I763007 I763007]+[http://partsregistry.org/Part:pSB4A3 pSB4A3];

-[http://partsregistry.org/Part:BBa_I763032 I763032]+[http://partsregistry.org/Part:pSB4A3 pSB4A3].

• Run on electrophoresis gel.
• [http://partsregistry.org/Part:BBa_I763007 I763007] and [http://partsregistry.org/Part:BBa_I763026 I763026] band extraction.
• O/N ligation for:

-[http://partsregistry.org/Part:BBa_I763026 I763026]+[http://partsregistry.org/Part:BBa_I763007 I763007];

-[http://partsregistry.org/Part:BBa_I763024 I763024]+[http://partsregistry.org/Part:pSB4A3 pSB4A3];

-[http://partsregistry.org/Part:BBa_I763032 I763032]+[http://partsregistry.org/Part:pSB4A3 pSB4A3];

-[http://partsregistry.org/Part:BBa_I763007 I763007]+[http://partsregistry.org/Part:pSB4A3 pSB4A3].

• We inoculate:

-[http://partsregistry.org/Part:BBa_I763031 I763031](low copy plasmid);

-[http://partsregistry.org/Part:BBa_I763016 I763016](low copy plasmid);

-[http://partsregistry.org/Part:BBa_I763032 I763032] (high copy plasmid).

10/09/07

• Transformation of:

-[http://partsregistry.org/Part:BBa_I763026 I763026] + [http://partsregistry.org/Part:BBa_I763007 I763007];

-[http://partsregistry.org/Part:BBa_I763024 I763024] (low copy plasmid);

-[http://partsregistry.org/Part:BBa_I763032 I763032] (low copy plasmid);

-[http://partsregistry.org/Part:BBa_I763007 I763007] (low copy plasmid).

• We inoculate in 6ml:

-[http://partsregistry.org/Part:BBa_I763031 I763031] (low copy plasmid);

-[http://partsregistry.org/Part:BBa_I763016 I763016] (low copy plasmid).

• Ligation for:

-[http://partsregistry.org/Part:BBa_I763016 I763016] (Eco/Spe)+GFP.(Eco/Xba).

10/10/07

• Miniprep of:

-[http://partsregistry.org/Part:BBa_I763016 I763016] (low copy plasmid);

-[http://partsregistry.org/Part:BBa_I763031 I763031] (low copy plasmid).

• Digestion for :

-[http://partsregistry.org/Part:BBa_I763031 I763031] with Xba/Pst1;

-[http://partsregistry.org/Part:BBa_I763016 I763016] (low copy plasmid) with Spe/Pst1;

• Run on electrophoresis gel and [http://partsregistry.org/Part:I763016 I763016] band extraction.
• Ligation for :

-[http://partsregistry.org/Part:BBa_I763016 I763016] (low copy plasmid)+GFP;

-[http://partsregistry.org/Part:BBa_I763024 I763024]+[http://partsregistry.org/Part:pSB4A3 pSB4A3];

-[http://partsregistry.org/Part:BBa_I763024 I763024]+[http://partsregistry.org/Part:BBa_C0012 C0012] (high copy plasmid).

• We inoculate in 6ml:

-[http://partsregistry.org/Part:BBa_I763031 I763031] (low copy plasmid);

-[http://partsregistry.org/Part:BBa_I763016 I763016] (low copy plasmid);

-[http://partsregistry.org/Part:BBa_I763026 I763026] + [http://partsregistry.org/Part:BBa_I763007 I763007](high copy plasmid);

-[http://partsregistry.org/Part:BBa_I763007 I763007] (low copy plasmid);

-[http://partsregistry.org/Part:BBa_I763024 I763024] (low copy plasmid).

10/11/07

• Stock preparation (500ul) of:

-[http://partsregistry.org/Part:BBa_I763031 I763031] (low copy plasmid);

-[http://partsregistry.org/Part:BBa_I763016 I763016] (low copy plasmid);

-[http://partsregistry.org/Part:BBa_I763026 I763026] + [http://partsregistry.org/Part:BBa_I763007 I763007](high copy plasmid);

-[http://partsregistry.org/Part:BBa_I763007 I763007] (low copy plasmid);

-[http://partsregistry.org/Part:BBa_I763024 I763024] (low copy plasmid).

• Miniprep of:

-[http://partsregistry.org/Part:BBa_I763031 I763031] (low copy plasmid);

-[http://partsregistry.org/Part:BBa_I763016 I763016] (low copy plasmid);

-[http://partsregistry.org/Part:BBa_I763026 I763026]+ [http://partsregistry.org/Part:BBa_I763007 I763007](high copy plasmid);

-[http://partsregistry.org/Part:BBa_I763007 I763007] (low copy plasmid);

-[http://partsregistry.org/Part:BBa_I763024 I763024] (low copy plasmid).

• Fluorescence test of:

-[http://partsregistry.org/Part:BBa_I763031 I763031] (low copy plasmid);

-[http://partsregistry.org/Part:BBa_I763026 I763026] + [http://partsregistry.org/Part:BBa_I763007 I763007] (high copy plasmid);

-[http://partsregistry.org/Part:BBa_I763007 I763007] (low copy plasmid).

• Control digestion of:

-[http://partsregistry.org/Part:BBa_I763031 I763031] with Xba/Pst1;

-[http://partsregistry.org/Part:BBa_I763016 I763016] with Xba/Pst1 and Spe/Xba;

-[http://partsregistry.org/Part:BBa_I763026 I763026] + [http://partsregistry.org/Part:BBa_I763007 I763007] with Xba/Pst1 and Spe/Xba;

-[http://partsregistry.org/Part:BBa_I763024 I763024] with Xba/Pst1;

-[http://partsregistry.org/Part:BBa_I763007 I763007] with Xba/Pst1.

• Run on electrophoresis gel and band extraction.
• Transformation of:

-[http://partsregistry.org/Part:BBa_I763019 I763019];

-[http://partsregistry.org/Part:BBa_I763024 I763024]+[http://partsregistry.org/Part:BBa_C0012 C0012];

-[http://partsregistry.org/Part:BBa_I763032 I763032].

• We inoculate

-[http://partsregistry.org/Part:BBa_I763019 I763019].

10/12/07

• Miniprep of:

-[http://partsregistry.org/Part:BBa_I763019 I763019].

• Digestion of:

-[http://partsregistry.org/Part:BBa_I763019 I763019] with Xba/Pst1;

-[http://partsregistry.org/Part:BBa_I763019 I763019] with Eco/Xba.

• Run on electrophoresis gel.
• [http://partsregistry.org/Part:BBa_I763019 I763019] (Xba/Pst1) and [http://partsregistry.org/Part:BBa_I763019 I763019] (Eco/Xba) band extraction.

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