From 2007.igem.org

An actual demonstration is determined to show the extensibility of our method. This demo system • is designed as simple as possible, without any "cool" logic function; • includes all the three logic gates, which constitute a three-level logic circuit; • shows that wires can cross and branch off (without interference); • is loaded on two plasmids, pSB1A3-I732998 and pSB1A3-I732999 ,and practically transformed into Top10 strain; • accepts aTc and AHL signals as inputs; • produces RFP and GFP signals as outputs; • can be "reset" by IPTG signal (i.e. both of the outputs are lightened when IPTG added in); • is expected to put out the results that accord with the truth value shown in Figure 2.

Logic Abstract of the Demo

We can see from this truth table that IPTG might result in both outputs lightened no matter aTc or AHL exists or not. (It is just like the response "8888..." on the screen when you reset one of your digital equipment, for example, a calculator.)

Actual System

The left three parts are loaded on [http://partsregistry.org/Part:BBa_I732998 pSB1A3-I732998], and the rest two parts are loaded on [http://partsregistry.org/Part:BBa_I732999 pSB1A3-I732999]. We've built up this system in one kind of TOP10 E.coli, but it seems that it cannot grow stably when aTc exist. (We have to use aTc at a high concentration since the high expression of LuxR.) I may not show the final results here before Oct. 26, but I'm trying my best to filter out a monoclonal strain for that. You will get the results in the coming presentation...