From 2007.igem.org

Summary The idea Promoter calibrator Biological comparator Electronic comparator Biological controller Lab Work Silico Work Results Adversities

In this section we try to summarize all the results obtained in the development of the project, this section has been divided in an experimental and a theoretical part. For a more extended description of the developed work we suggest to visit the sections in silico work and lab work.

Experimental achievements

We have obtained many new constructions, some of them sequenced and

Theoretical development

• An effective model to describe and analyze the behaviour of our system has been developed.

• This model is composed by the following set of five equations:

• The parameters of the model have unknown values, as a consequence of this, the values taken by Elowitz were selected as a first approximation to their real value.

• According to this an stability and sensibility analysis of the EcoliRuler according the different parameter values has been been performed.

• After developing the facs measurements, in principle we should be able to give a better estimation of the values of the model parameters. So, preparing that part of the project in advance, a strategy to determine those constants has been designed.