Bologna University/Plates preparation

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1. Autoclave LB medium with 2% agar.
2. Cool at 50 °C to prevent agar polymerization.
3. Before preparing plates add antibiotic (Ampicillin 1000x [ ] or Kanamicin 200x [ ]).
4. Put about 20ml of medium per plate.
5. Leave it solidify and store at 4°C.


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