USTC/RepressionMatrixFromLiterature

From 2007.igem.org

The Repression Matrix comes from other's previous work results[7,8,9]. However, we hardly get an 'ORM' because the cross repression are obvious according to this results. i.e. we may not obtain stable Repressor-Promoter Pairs as our wires with high fidelity.

Figure I The same with the former RM that red color represents a high repression while blue represent reverse. This Matrix are not directly from literatures but from the data by previous research work.

References

1. Lehming, N.; Sartorius, J.; Niemöller, M.; Genenger, G.; v Wilcken-Bergmann, B. & Müller-Hill, B. (1987), 'The interaction of the recognition helix of lac repressor with lac operator.', EMBO J 6(10), 3145--3153.

2. Lehming, N.; Sartorius, J.; Oehler, S.; von Wilcken-Bergmann, B. & Müller-Hill, B. Recognition helices of lac and lambda repressor are oriented in opposite directions and recognize similar DNA sequences. PNAS, 1988, 85, 7947-7951

3. Sartorius, J.; Lehming, N.; Kisters, B.; von Wilcken-Bergmann, B. & Müller-Hill, B. (1989), 'lac repressor mutants with double or triple exchanges in the recognition helix bind specifically to lac operator variants with multiple exchanges.', EMBO J 8(4), 1265--1270.