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BOL: Bacteria OnLine

University of Cambridge iGEM 2007

Introduction

????Blurb about BOL????These are the projects we are undertaking this year:

Standardising the Agr signalling system

The Accessory Gene Regulator (Agr) system in S. aureus is an oligopeptide-based quorum sensing mechanism. We aim to convert it into a reusable interbacterial signalling system for communication amongst and between both Gram-positive species (B. subtilis) and Gram-negative bacteria (E. coli), based on controllable secretion and detection of the signalling peptide AIP.

For more information, see Signalling project description.

Bacillus subtilis SynBio Chassis

Most synthetic biology so far has involved Gram-negative bacteria, which are more widely used in molecular biology research; however, the biotechnology industry relies on Gram-positive bacteria. We are trying to make a "chassis" out of Bacillus subtilis, a common Gram-positive bacterium, into which genetic engineers can add BioBrick parts and control circuits.

For more information, see Bacillus subtilis SynBio chassis

PoPS amplifier

We aim to build a standard amplifier which can be used in any synthetic (BioBricked) system, which would take in a standard PoPS input and give a PoPS output of a known amplification factor.

For more details, have a look at the amplifier project.

[http://www.ccbi.cam.ac.uk/iGEM2007/index.php/Main_Page Go to the Cambridge iGEM 2007 team's official wiki!] (with more details about the team and how we came up with this idea)

[http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2007&group=Cambridge BioBricks we have contributed to the Registry]

Meet the team!


The Cambridge iGEM 2007 team!

Front row, from left: Narin Hengrung, Yi Han, David Wyatt

Second row, from left: Zhizhen Zhao (Jane), Stefan Milde, Dmitry Malyshev, Xinxuan Soh (Sheila)

Third row, from left: Yi Jin Liew, John Crowe (faculty), Lovelace Soirez, Stephanie May, Yue Miao

Back row, from left: James Brown (PhD student mentor), Jim Haseloff (faculty supervisor), Gos Micklem (faculty supervisor)

Not in photo: Jim Ajioka (faculty supervisor), Lorenz Wernisch (faculty), Tony Southall (PhD student)

The mailing list for the whole Cambridge iGEM2007 team is igem2007 [at] ccbi.cam.ac.uk

Sponsors

We are extremely grateful to the following organisations for their support of our project:

Major sponsors

[http://www.cam.ac.uk https://static.igem.org/mediawiki/2007/9/95/Cambridge_UofC_coat_of_arms.png]     [http://www.dna20.com https://static.igem.org/mediawiki/2007/1/16/Cambridge_DNA2_0_logo.png]

Other sponsors

  • The Isaac Newton Trust-funded UROP scheme
  • EU SynBioComm programme


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