From 2007.igem.org

Peking	The Projects	The Team	Misc & Fun	[http://openwetware.org/wiki/IGEM:Peking/2007 Peking OWW]	Thanks

Self-differentiated Bacterial Assembly Line

Our projects concerns with the ability for bacterial cells to differentiate out of homogeneous conditions to achieve the division of labor. We want to design devices conferring host cells with the ability to spontaneously form cooperating groups and to take consecutive step even when the genetic background and environmental inputs are identical. To break the mirror in such homogeneous condition, we need two devices respectively responsible for temporal and spatial differentiation. The implementation and application of such devices will lead to a picture of future bioengineering where complex programs consisted of sequential steps (structure oriented programs) and cooperating agencies (forked instances of a single class, object and event oriented) can be embedded in a single genome. The expression of such a genome pre-programmed both temporally and spatially both resembles the differentiation process of multicellular organism and a assembly plant built out of a blueprint.

Hop Count with Conjugation

Hop counting is a cell-cell communication system in which the signals transfered among the cells record the number of times of transfer. we have adapted natural conjugation systems as the communication device, and the conjugative plasmids as the vector for the transfered signal. With the rolling-cycle replication of conjugation, independent tandem oriT (BBa_J01002 and more) pairs can be inserted into the same plasmids, losing one tandem region at each single conjugation event. Sequential conjugation and deletion of the different oriT pairs can be achieved by fine control of the expression of the cis-acting elements of the independent conjugation systems.Besides intellectual interests, these systems are the core parts of controllable and encoded local cell-cell communication, which can be used in potential pattern formation and material weaving applications.

Push-on-push-off Switch

We are going to engineer the E. coli cell to behave as a push-on push-off switch. We have designed a gene regulatory circuit that senses Ultra-Violet light (UV) irradiation as input signal. Under intermittent irradiation, two reporter genes are to be induced alternately. The circuit can be divided into two associated parts: a bistable switch and a NOR gate. The NOR gate is controlled by the bistable switch and the input signal. On the other hand, the output of the NOR gate can switch the bistable state from one to the other. Thus far, we have constructed a series of NOR gates and one bistable switch.