From 2007.igem.org

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Testing Floatation

• Growing up cells from glycerol stocks overnight in 5mL LB amp
• Prepare 250ml conical shaker flasks by cleaning and autoclave.
• Add 50mL of LB Amp to each of two flasks.
• Add 1M IPTG to one flask to make final concentration of 1mM IPTG, Lable with I.
• Transfer 1mL of overnight culture to each flask.
• Grow overnight at 37degC on a shaker.
• Next morning transfer to 50ml falcon tubes.
• Spin down the cells gently 25minutes at 1000rpm in Eppendorph 5810R centrifuge.
• Poor off and discard the LB, maintaining the pellet and a residual ammount of LB 1ml about.
• Replace LB with 50ml of 10g/L NaCl filtered through a 0.22um filter.
• Resuspend the cells by shaking.
• Leave at room temperature and observe the settling over the next week.

Example pNL29(orininal plasmid) and Tube(64) the IPTG promoted version of BBa_I750016

Image:Melbourne-float1 11am 18-10-2007.JPG