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Project overview (Short description)
Background & papers referenced
Original Plan
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Project
This project aims to use light to form a solid fluorescent mass of E. coli where two light beams intersect in a suspension of cells. We've called our building system "Coliforming".
Motivation
Possible applications in building complex scaffolds in tissue engineering.
Requirements
The system requires six biological components.
- Red photosensor
- Blue photosensor
- AND gate
- GFP fluorescent reporter
- Promotable surface expression of cadherins
- Gas vesicle expression to produce neutrally bouyant bacteria
High Level System Design
- Induce or constitutively express gas vesicles for neutral bouyancy
- If Red & Blue light detected, then promote adhesion molecules.
Achievements
- Produced bouyant bacteria biobrick.
- Produced parts of other biological components required.
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