From 2007.igem.org

Promoter Test Constructs

To test each of the four xyl promoters, each is placed in a construct followed by a ribosome binding site, the reporter green florescent protein, and a terminator. The strength of each is measured by gfp emission.

This construct uses the xylF and xylA facing promoters that includes the CRP-cAMP binding region.

This construct uses the xylF and xylA facing promoters that do not include the CRP-cAMP binding region.

After each of the four promoter constructs are tested individually, the two which include the CRP-cAMP are changed so that a gene expressing the mutant CRP* is included.

This construct uses the xylF and xylA facing promoters that do not include the CRP-cAMP binding region followed by the CRP* gene.