Chiba/Sandbox/Home2

From 2007.igem.org

Project Overview

Our iGEM project is to make a Marimo-ish gathering of bacteria. Marimo is a green spherical shaped algae (shown in Fig.1), which is a popular living organism in Japan as a National Treasure.(<because of its shape?) Motivation.
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Our marimo bacteria requres as follows:

Methods

Achievements Overview

Making Affinity Tags

Fig. The short peptide with six histidine (“His-Tag”) was inserted into the fliC D3 domain.

1. Make the affinity tag by inserting the his-tag into the flagellar fillament.
   • Sequence confirmed
   • Swarm confirmed
   • Flagella strained with anti-flagella antibody
   • Phenotype confirmed
   • Affnity confirmed

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Making Communication Module

1. Making Senders
   • AHL > GFP generator (constitutive aiiA) : BBa_T729006
   • AHL > GFP & aiiA generator : BBa_I729005
   • Sensitive AHL > GFP generator : BBa_I729004

[edit] Future Work inverter-aiiA

1. • 1.---
   • 2.---
2. Making Receivers
   • 1.---
   • 2.---

Controlling Size

1. Improve Sender
   • 前駆体合成酵素metKを入れてみた．＞結果？
2. Improve Receiver
   • LuxRの感度が良くなるmutation（論文より）を入れてみた．＞結果
3. Localize AHL
   • aiiAその１の回路（〜〜になるはず）＞結果
   • aiiAその２の回路
   • その３

Making Marimo

Remaining Task(Work)

Our Goal

Future