June 27

From 2007.igem.org

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Experiments

• Dilutions of pT luxI.cfp made for equivalence.

• pT.luxI.cfp inoculated into LB for Open Loop[A] (morning)

• pT.luxI.cfp and K12Z1 inoculated in LB for Open Loop[B] (evening)

• pLac.cfp Inoculated for equivalence.

• Inoculated into 50 mL Glu M9 with 20 ng/mL aTc (20*50=1 ug == 100 uL of 10 ug/mL)

pT LuxI.C: 2 flasks - 1 ul/mL == 50 uL
                    0.1 ul/mL == 5 uL
    
    K12z1: 1 flask  - 2 ul/mL == 100 uL

• Inoculated pL luxR yfp.pR cfp and K12Z1 in LB for Open Loop[A]

• pLac.Cfp dilutions done (14+2+4=20 tubes)

Microscopy

• OD was measured and pT luxI.C imaging done for equivalence.

• pL.luxR.yfp imaging done for equivalence.

Analysis