From 2007.igem.org

Beta portion
pcr CA1154F/G00101 on 9145-I716063 (575 bp)
Product is ADtemp1b
Sub ADtemp1b into pTRC plasmid with (Bgl2/XhoI)
Product is ADtemp2b
pcr CA1099F/D58 on ADtemp2b (3465 bp)
Product is ADtemp3b
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Alpha portion
Pcr CA1150F/CA1099R on I716077 ()
Product is ADtemp5b
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Digest ADtemp3b (BsaI/BamHI)
Digest ADtemp5b (BsaI/BglII)
Ligate, product is: I716075 (Best all around)