From 2007.igem.org

University of Waterloo iGEM Team

Contents 1 Our Team 2 Our Design 2.1 The Inputs 2.2 The Logic Gates 3 Supporters

Our Team

The UW iGEM team is a very interdisciplinary group. Our team members span the four faculties of Science, Mathematics, Engineering, and Applied Health Sciences and include the programs of Biology, Health Studies, Computer Science, Bioinformatics, Computer Engineering, Electrical Engineering, Chemical Engineering, and Mathematical Physics at undergraduate and graduate levels. Even our professor advisors are cross-appointed to two other faculties. Our diverse backgrounds bring together a wide range of skills and ideas to the iGEM project. iGEM is giving us the opportunity to learn.

Our Design

Our project is a light-controlled 1-bit adder.

The Inputs

We will be using red light as one of the inputs and blue light as the other. The red light will be detected by the previously used Cph1/EnvZ fusion protein ([http://partsregistry.org/Part:BBa_I15010 BBa_I15010]).

The blue light input will be derived from the Myxococcus xanthus carotenogenesis system, a list of relevant references can be found below. In the event this input should prove difficult to implement, we will use a chemical input instead.

Blue-light References:

1. Moraleda-Muñoz A, Pérez J, Fontes M, Murillo FJ, Muñoz-Dorado J. (2005) Molecular Microbiology 56 (5):1159-68
2. Browning DF, Whitworth DE, Hodgson DA. (2003) Molecular Microbiology 48 (1):237-251
3. Whitworth DE, Bryan SJ, Berry AE, McGowan SJ, Hodgson DA. (2004) Journal of Bacteriology 186 (23):7836-46
4. Whitworth DE, Hodgson DA. (2001) Molecular Microbiology 42 (3): 809-819
5. López-Rubio JJ, Elías-Arnanz M, Padmanabhan S, Murillo FJ. (2002) The Journal of Biological Chemistry 277 (9):7262-70

We have chosen to use light as an input for many reasons: it does not require altering the media, it can be turned on and off rapidly, and it has extremely high resolution and accuracy.

The Logic Gates

In order to have a functional adder, we are designing an AND gate as well as an XOR gate.

Supporters

We would like to thank:

[http://www.science.uwaterloo.ca Faculty of Science] — [http://www.math.uwaterloo.ca Faculty of Math] — [http://www.engineering.uwaterloo.ca Faculty of Engineering]

[http://www.weef.uwaterloo.ca Waterloo Engineering Endowment Fund] — [http://www.mef.uwaterloo.ca Mathematics Endowment Fund]