BerkiGEM2007-AustinConstructionFile25
From 2007.igem.org
Alpha:
PCR CA1150/CA1099R on I716077 (1727 Bp)
Digest (Bsa1/Bgl2) and ligate with beta portion.
Beta:
PCR CA1099R/D58 on Part Ptrc-I716082
Digest (Bsa1/BamH1) and ligate with alpha portion.
Product is pTRC-I716075 (Best all around)