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Contents 1 Week 1 1.1 25 June 2007 1.1.1 Transformation 1.2 26 June 2007 1.3 27 June 2007 1.4 28 June 2007 1.5 29 June 2007 1.6 30 June 2007 2 Week 2 2.1 2 July 2007 3 Now 3.1 3 July 2007 3.2 4 July 2007 3.3 5 July 2007 3.4 6 July 2007 3.5 Week 3 3.5.1 9 July 2007 3.5.2 10 July 2007 3.5.3 11 July 2007 3.5.4 12 July 2007 3.5.5 13 July 2007 3.6 Week 4 3.6.1 16 July 2007 3.6.2 17 July 2007 3.6.3 18 July 2007 3.6.4 19 July 2007 3.6.5 20 July 2007 3.7 Week 5 3.7.1 23 July 2007 3.7.2 24 July 2007 3.7.3 25 July 2007 3.7.4 26 July 2007 3.7.5 27 July 2007 3.8 Week 6 3.8.1 30 July 2007 3.8.2 31 July 2007 3.8.3 1 Aug 2007 3.8.4 2 Aug 2007 3.8.5 3 Aug 2007

Week 1

25 June 2007

Prepared LB agar plates.

Transformation

1. resuspended the following from Registry plates:

• • BBa_I15008 - ho1 (P2 21A)
  • BBa_I15009 - PcyA (P2 21C)
  • BBa_R0084 - OmpR positive promoter (P1 11H)

1. 1. Punctured foil with pipette tip.
   2. Resuspended in 15uL ddH2O.
   3. Stored in-20 (after taking 1uL for transformation).
2. Transformed into competent DH5alpha cells with shorter incubationtimes as follows:

• • 30min on ice after DNA addition
  • 10min on ice after heat shock
  • 30min at 37degrees with LB

26 June 2007

27 June 2007

28 June 2007

29 June 2007

30 June 2007

Week 2

2 July 2007

Now

3 July 2007

4 July 2007

5 July 2007

6 July 2007

Week 3

9 July 2007

10 July 2007

11 July 2007

12 July 2007

13 July 2007

Week 4

16 July 2007

17 July 2007

18 July 2007

19 July 2007

20 July 2007

Week 5

23 July 2007

24 July 2007

25 July 2007

26 July 2007

27 July 2007

Week 6

30 July 2007

31 July 2007

1 Aug 2007

2 Aug 2007

3 Aug 2007