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Contents 1 Week 1 1.1 25 June 2007 1.1.1 Transformation 1.2 26 June 2007 1.2.1 Transformation from Monday 1.2.2 Transformation 1.2.3 Streak plates 1.3 27 June 2007 1.4 28 June 2007 1.5 29 June 2007 1.6 30 June 2007 2 Week 2 2.1 2 July 2007 3 Now 3.1 3 July 2007 3.2 4 July 2007 3.3 5 July 2007 3.4 6 July 2007 3.5 Week 3 3.5.1 9 July 2007 3.5.2 10 July 2007 3.5.3 11 July 2007 3.5.4 12 July 2007 3.5.5 13 July 2007 3.6 Week 4 3.6.1 16 July 2007 3.6.2 17 July 2007 3.6.3 18 July 2007 3.6.4 19 July 2007 3.6.5 20 July 2007 3.7 Week 5 3.7.1 23 July 2007 3.7.2 24 July 2007 3.7.3 25 July 2007 3.7.4 26 July 2007 3.7.5 27 July 2007 3.8 Week 6 3.8.1 30 July 2007 3.8.2 31 July 2007 3.8.3 1 Aug 2007 3.8.4 2 Aug 2007 3.8.5 3 Aug 2007

Week 1

25 June 2007

Prepared LB agar plates.

Transformation

1. resuspended the following from Registry plates:

• • BBa_I15008 - ho1 (P2 21A, Kan)
  • BBa_I15009 - PcyA (P2 21C, Kan)
  • BBa_R0084 - OmpR positive promoter (P1 11H, Amp)

1. 1. Punctured foil with pipette tip.
   2. Resuspended in 15uL ddH2O.
   3. Stored in-20 (after taking 1uL for transformation).
2. Transformed into competent DH5alpha cells with shorter incubation times as follows:

• • 30min on ice after DNA addition
  • 10min on ice after heat shock
  • 30min at 37degrees with LB

26 June 2007

Transformation from Monday

• Transformation of BBa_I15008 and BBa_I15009 failed. No colonies on plates
• Small number of colonies on BBa_R0084 plate.
• Placed in cool room

Transformation

1. Resuspended the following parts in 15uL:

• BBa_B0034 (RBS, plate 1 3O, Amp)
• BBa_C0051 (C1 repressor, plate 1 5G, Amp)
• BBa_B0010 (Terminator, plate 2 3P, Amp)
  • Think some DNA may have remained in wells

1. Transformed into competent DH5alpha cells from Joe

Streak plates

1. Streaked the following cells:

• PJS010 (from solid agar, Amp)
• Fusion protein (from glycerol stock, Kan)
• BBa_I15010 (from solid agar, Kan)

27 June 2007

28 June 2007

29 June 2007

30 June 2007

Week 2

2 July 2007

Now

3 July 2007

4 July 2007

5 July 2007

6 July 2007

Week 3

9 July 2007

10 July 2007

11 July 2007

12 July 2007

13 July 2007

Week 4

16 July 2007

17 July 2007

18 July 2007

19 July 2007

20 July 2007

Week 5

23 July 2007

24 July 2007

25 July 2007

26 July 2007

27 July 2007

Week 6

30 July 2007

31 July 2007

1 Aug 2007

2 Aug 2007

3 Aug 2007