Bangalore
From 2007.igem.org
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The official wiki of the NCBS iGEM 2007 Team |
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Our team at the National Centre for Biological Sciences, Bangalore comprises undergraduate and graduate students from diverse backgrounds of science. We have gathered together to collectively investigate Multistability and Hysteresis in the Combinatorial Synthetic Vibrio Quorum Sensing Circuits designed by us. We have designed a Mathematical Model for our circuits and are simultaneously carrying out a corresponding set of experiments using the bacterial strain E.coli-K12Z1. |
| [http://www.ncbs.res.in/ National Centre for Biological Sciences, Bangalore] |
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| Bangalore | The Team | The Mission | e-Notebook |
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It has been shown (Angeli et al) that for a class of feedback systems of arbitrary order, the stability properties and bifurcation diagram of the system can be deduced mathematically from how the system behaves when feedback is blocked. The system is guaranteed to be bistable for some range of feedback strengths provided the feedback-blocked system is monotone and shows a sigmoidal characteristic.
We have taken the Vibrio Quorum Sensing System. In this system, when the population density (and hence the concentration of autoinducer produced) crosses a certain threshold, the expression of a set of genes that is required for bioluminescence is turned on. The production of the autoinducer is under the control of a gene, the expression of which involves positive feedback. The transcriptional regulator, LuxR* governs the expression of the promoter, pR and we use CFP as the reporter here. Since the concentration of LuxR* depends on the concentrations of both AI and LuxR, we have a remarkable handle on the feedback of this system.
Note that this is a positive feedback system and its open loop can be closed in two different ways as shown in fig.
Now we plan to check for the bistability of a similar synthetic genetic circuit constructed by us using various constructs.
Our approach is to conduct a set of open loop experiments on the bacterial strain E-coli K12Z1. We vary the concentrations of AI and LuxR and get the I/O characteristic curves over a range obtained by varying the concentration of the corresponding inducers (aTc and IPTG). We quantify the expression levels for the genes at different stages by measuring the fluorescence intensities of CFP and YFP reporter proteins in the constructs using Fluorescence Microscopy.
The sigmoidal family of curves obtained from these open loop experiments when intersect with the superimposed equivalence line will give the steady states for the system. And thus the stability of these states will be concluded thereafter on the theorem proposed by Angeli et al.
For a detailed description of our project, go to The Mission
Bangalore iGEM 2007
