From 2007.igem.org

Preparation of a stock of phage on w121:

David B:

• Filtration of the Cacl2 and MgSO4 stocks
• Launch a culture:
  • 15ml LB
  • 225µl Cacl2
  • 450µl MgSO4
  • 90µl DAP
  • 150µl W121 from an ON culture
• Let grow until OD=0.2 (~1h)
• Add 100µl P1 (stock 10^10/ml)
• Incubate 2h
• Add 200µl Chloroform
• Vortex, centrifuge (15min @ 3000rpm)
• Recover the supernatant + add 200µl Chloroform = Stock keep at 4°C

Titration of bacteriophages P1

Nicolas C. We measure the strength of former P1 preparation :

• The stock prepared the 3.7.7
• The stock prepared the 8.7.7

See protocols for details.

Culture of FtsZ TS

David B We want to isolate a good clone of the strain FstZ TS
From the plate of FtsZ TS84 121:
isolation of 6 clones of the 121.1 on LBA+tet incubated at 42°C + Culture ON at 30°C
If one clone do not grow at 42, we'll use it to do the transduction