Composite part of RBS (pBca1101-I716051-kan cassette) + hemA from R.capsulatus Digest pBca1101-I716051 (EcoR1/BamH1, 2904+1065, S) Sub into pBca9145-I716151 (EcoR1/Bg1II, 3275+9, L) Product is pBca9145-I716156