From 2007.igem.org

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Contents 1 Biobricks Revived 2 Lab Diary 2.1 Thu 12th July 2.2 Fri 13th July 2.3 Mon 16th July 2.4 Tue 17th July 2.5 Wed 18th July 2.6 Thu 19th July 2.7 =Fri 20th July

Biobricks Revived

This is a list of the biobricks we have revived from the registry

Brick	Description	Well	Used for
[http://partsregistry.org/Part:BBa_J04430 BBa_J04430]	IPTG induced GFP	Plate 2, 1L	Test biobrick revival process
[http://partsregistry.org/Part:BBa_E0241 BBa_E0241]	PoPS --> GFP	Plate 2, 15L	For use in dif experement 1
[http://partsregistry.org/Part:BBa_J45700 BBa_J45700]	Wintergreen Scent	Plate 3, 7B	Place into lacto bacillus
[http://partsregistry.org/Part:BBa_E0422 BBa_E0422]	fast degrading ECFP	Plate 1, 11G	For use in dif experement 1

Lab Diary

Thu 12th July

• PTG induced GFP revived ([http://partsregistry.org/Part:BBa_J04430 BBa_J04430])

Test biobrick revival process

Fri 13th July

• Alfalfa Seeds Planted (in Agar)

Growing alfalfa plants, to get leaves from which to extract DNA for the caffeic acid-3-o-methyltransferase gene (part of the vanillin synthesis pathway)

• Biobricks [http://partsregistry.org/Part:BBa_E0241 BBa_E0241] and [http://partsregistry.org/Part:BBa_J45700 BBa_J45700] revived

Mon 16th July

• Begin DNA extraction from Bamboo

Test DNA extraction process from plants

• Prepared mini preps for GFP and mint genes

Tue 17th July

• Alfalfa Transferred to compost

• DNA containing GFP and mint genes extracted from E.Coli

Wed 18th July

• Digest GFP and mint mini preps and ran on gel
• Biobrick [http://partsregistry.org/Part:BBa_E0422 BBa_E0422] revived

Thu 19th July

• Repeat digest of mini preps with more DNA

=Fri 20th July

• Revived Pseudamonas Fluorescens from freeze dried culture

Used to retrieve frs and ech genes in vanillin pathway