From 2007.igem.org

AustinDay 01:42, 31 July 2007 (EDT)

• Can we create an injectable bacterium that can make you sober up quickly? I dunno, let's figure it out.
• I think we want Class I alcohol dehydrogenases. They have a lower Km, however there are many different polymorphisms of class I ADHs with different Kms, it appears as though having many different types would be the best/most biomimetic approach.
• The first step catalyzed by the alcohol dehydrogenase is the limiting step right now.
• The second enzyme, acetaldehyde dehydrogenase will break down the very toxic acetaldehyde to acetic acid, which you pee out. This is the pathway that ~90% of ethanol is metabolized by.
• This might be a good place to use John's scaffolding technology to fuse the two enzymes together to reduce the concentration of the toxic intermediate? That'd be fun.