Mechanism II

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Yeast can produce riboflavin itself, but it can only supply its own metabolism without accumulation. There are 6 genes related to the riboflavin production. The patyway of riboflavin synthesis is showed below:

Figure 4 the riboflavin synthesis pathway

We propose to change the prmoters related to riboflavin synthesis of Yeast to strong promter ADH1. The two-step-gene replacement method is used in our project. Take RIB1 as an example. Take the RIB1gene as three regions: region1, region2 and region2 (figure5)

Figure 5 the regions in RIB1 gene
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