Week 3

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07/17/07


  • We amplified some bio- bricks of interest for our project from the IGEM plates. We also amplified a plasmid (BBa_ J04430) to test the GFP fluorescence detection with our experimental set up.


7/18/07
  • We picked a colony in 5 ml of LB medium from each plate and incubated overnight
  • We picked a colony and grew it till a OD 0.4 – 0.6. We added 1 mM IPTG to induce GFP expression.