Week 3
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Revision as of 13:25, 9 August 2007 by Francesca.bugane2 (Talk | contribs)
- 07/17/07
- We amplified some bio- bricks of interest for our project from the IGEM plates. We resuspended and transformed:
-BBa_ J04430 to test the GFP fluorescence detection with our experimental set up -BBa_J04431 to test the GFP (+LVA) half-life since we needed a GFP with a short one for our project.
- We verified that the GFP (+LVA) displays...
- BBa_J04500, the PLac promoter inducible by IPTG;
- BBa_J04631, the GFP (+LVA) protein.
- 7/18/07
- We picked a colony in 5 ml of LB medium from each plate and incubated overnight.
- We picked a colony and grew it till a OD 0.4 – 0.6. We added 1 mM IPTG to induce GFP expression.
