From 2007.igem.org

Design Issues

• About insulin
  • activate insulin
  • transport insulin (Dr. Chang)
  • test insulin (functional assay)
  • inhibit insulin
• About glucose-sensor
  • fusion construct
• About system verification in human cell (A ken)
  • co-culture with bacterium
  • commercial insulin response (positive control)
  • insulin in media response
  • glucose response and measure