Melb:Plan:GFP Fluorescent reporter

From 2007.igem.org

Revision as of 07:29, 11 August 2007 by Patricia (Talk | contribs)
(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)

LacZ reporter

  1. Ligate LacZ coding region to gentamycin RBS part. (Done?)
  2. Transform and select for appropriate construct using Kan/Gen plate. (Done?)
  3. Liquid culture and screen via restriction digest (EcoRI and SpeI), digest double terminator (P1 1G) simultaneously (EcoRI and XbaI) (Monday 13/08/07)
  4. Make glycerol stock of RBS-LacZ containing cells. (Monday 13/08/07)
  1. Ligate the RBS-LacZ construct into double terminator plasmid. (Monday 13/08/07)
  2. Transform ligation and select for construct
Retrieved from "http://2007.igem.org/wiki/index.php/Melb:Plan:GFP_Fluorescent_reporter"