Experiments

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The official wiki of the NCBS iGEM 2007 Team
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The NCBS iGEM 2007 Experiments

The following is the record of all the experiments done by us, each followed by graphs obtained by analysis of the corresponding microscopy and flow cytometry data.

Note that in flow cytometry a signal obtained from a filter does not exactly correspond to CFP or YFP amount inside a cell; when cells express both the proteins. This is because of spectral overlap of their excitation and emission spectra. We came up with a mathematical method to separate CFP and YFP from autofluorescence and noise. Click here for details about this mathematical tool for correction.

[http://www.ncbs.res.in/ National Centre for Biological Sciences, Bangalore]


Bangalore The Team The Mission Experiments e-Notebook


Contents

Experiment

Equivalences

  • pL.Cfp

PLC dup.png

  • pT.luxI.Cfp

PTIC dup.png

  • pL.luxI.Cfp

PLIC dup.png

  • pL.luxR.Yfp

PLRY dup.png

Open loops

  • pT.luxI.Cfp::pL.luxR.Yfp.pR.Cfp (0 ng/ml aTc)

Openloop 0.png

  • pT.luxI.Cfp::pL.luxR.Yfp.pR.Cfp (1 ng/ml aTc)

Openloop 1.png

  • pT.luxI.Cfp::pL.luxR.Yfp.pR.Cfp (5 ng/ml aTc)

Openloop 5.png

  • pT.luxI.Cfp::pL.luxR.Yfp.pR.Cfp (10 ng/ml aTc)

Openloop 10.png

  • pT.luxI.Cfp::pL.luxR.Yfp.pR.Cfp (20 ng/ml aTc)

Openloop 20.png

  • pT.luxI.Cfp::pL.luxR.Yfp.pR.Cfp (50 ng/ml aTc)

Openloop 50.png


Closed loops

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