From 2007.igem.org

09/03/07

• Miniprep for:

-R0040+S0100;

-R0040+S03520;

-I763005+I763015;

-J52034+E0022;

• Digestion for:

-R0040+S0100 with Eco/Spe;

-R0040+S03520 with Eco/Spe;

-I763005+I763015 with Spe/Pst1;

-J52034+E0022with Spe/Pst1;

-B0015 with Eco/Xba;

• Band extraction;
• Ligation for:

-I763025+B0015(I763026);

-I763024+B0015(I763030);

-I763016+I763020(I763019);

-I763009+P0412(I763021);

-I763009+S0100(I763023);

-I763009+S03520(I763022);

-R0051+I763020(I763011);

09/04/07

• There are colonies for every ligations.
• Meeting.

09/05/07

Miniprep for:

-I763016+I763020(I763019);

-I763025+B0015(I763026);

-I763024+B0015(I763030);

-R0051+I763020(I763011);

-I763009+P0412(I763021);

-I763009+S03520(I763022);

• Digestion for:

-I763019 with Eco/Xba;

-I763026 with Eco/Spe;

-I763030 with Eco/Spe;

-I763011 with Xba/Pst1;

-R0051+I13507(I763007) with Xba/Pst1;

-I763021 with Eco/Spe;

• Control digestion for I763019 with Eco/Pst1;
• Band extraction for

-I763019;

-I763026;

-I763011;

-R0051+I13507(I763007);

• New control digestion for:

-I763019 with Xba/Spe;

-I763019 with Xba/Pst1;

-I763021 with Eco/Spe;

-I763026 with Eco/Spe;

-I763030 with Eco/Spe;

• Band extraction for I763019 (digested with Xba/Spe and with Xba/Pst1), I763026;
• Ligation O/N for:

-I763026+I763019(I763028);

-I763026+I763004(I763027);

09/06/07

Digestion for:

-I763019 with Eco/Xba;

-I763021 with Eco/Spe;

-I763026 with Eco/Spe.

• Band extraction from electrophoresis gel for I763019 and I763026.
• Ligation for I763027 with new extracts.
• Transformations for the new ligations.

09/07/07

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