NYMU Taipei/Lab Notes/2007 9 11

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  • Fresh back
    • two different PCR (INS_A and INS_B) have been done by 海威 (9/5, 2007) and 惇茹 (9/8, 2007)
    • we had failed three times for construction of pOmpC + INS_A using 惇茹's INS_A and following three different ligation protocol
      • insert (1 uL) vs. vector (1 uL)
      • insert (2 uL) vs. vector (1 uL)
      • insert (3 uL) vs. vector (1 uL)
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