From 2007.igem.org

Contents 1 5:30 Action points from last meeting: 2 5:35 Status Reports: (5 minutes each including any discussion) 3 5.55 New Agenda Items: 4 6:15 End 5 Igem Administrative Timetable: 6 Documentation wiki status:

5:30 Action points from last meeting:

• Pat & Alisa: LacZ reporter plan - complete

• Jan: Plan for blue light on wiki by 17th August- Done but needs more work: couldn’t get anyone else to implement it based on this!

• Phil: re prism :Edmund optics:VIS60 Schott veril linear variable interference filter SGD$1600 25mm wide 60mm long linear 400nm-700nm (contact sheet)

N47-279 eqilateral prism 35mm long 35mm face SGD$162

• Phil: Chase Status of #BBa-M30109 & #BBa-V1012 - Arrived

• Craig: Alkaline lysis technique for miniprep to be documented

• Craig: Protein extraction technique –done sent to Phil by email.

• Cheng: Daisy to produce competent cells of DB3.1 and Enz- strains: Alisa will set up plates for Daisy to work from by monday so we will have a store of competant cells by Tues/Wed

• Chris: Gas vesicle expression experiment by 21/8/07.-Done

5:35 Status Reports: (5 minutes each including any discussion)

• Alisa: Ligation experiments progress
  • Craig ligated the ompR promoter to the RBS-LacZ-Ter and c1 inverter, confirmation digest and gel to be done on saturday and parts sent for sequencing confirmation on Monday.
  • Additional ligations to be set up and confirmed during the week: OmpR test harness(Ompr-RBS-LacZ-ter) to be ligated behind M30109, Ompr-c1 inverter to be ligated behind M30109.

• Pat & Alisa: Red light receptor progress/design/plan- needs?
  • these include to above ligations. We need to work out how we are going to test these parts

• Jan: Blue light receptor design/plan progress - needs? (Absent from meeting)

• Phil: Gas vesicles progress/design/plan - needs?
  • Progress good, now on round 3 of mutagenesis (3 plates x2).
  • Need miniprep of six colonies on Monday and Digest with PstI, I will pick colonies Sunday.
  • Buoyancy experiment confused re IPTG, -IPTG,+IPTG but definitely differences. See attached photos at 1hour,6hours,18hours,96hours and last 3 at 126hours
  • Other parts required from kit->glycerol stocks: B034 (P1-3O) , B0015 (P1-1I or P3-3O), J61034 (P4-8F or 8H)

5.55 New Agenda Items:

• Phil: Administrative Timetable: (8 Weeks to go)

• Final Team roster and Jamboree attendance fees due. Who is going? Jan & Phil perhaps, others??? Need to find out state of funds so that we can work out how many people we are able to send
• Chris: Next years Comp Plan/preparations - same state as last week

• Any other business

• Next meeting: <b/> 28th August (Friday) 5.30PM.

6:15 End

Igem Administrative Timetable:

1 September	Final team rosters due.
1 September	Jamboree attendance fees due $100USD per team member.
26 October	Project and part documentation due DNA received USA.
26 October	BioBrick Part DNA received by the Registry.
3-4 November	iGEM Competition Jamboree, MIT, USA.

.

Documentation wiki status:

• • Priority should be on plans and experimental report analysis and conclusions-----Alisa .

• • If only Pat & Alisa doing lab work not much documentation needed.

• • Equipement:

• • • MissingLas3000 method (in lab notebook)

• • • Missing DNA measurement protocol (eppendorf)

• • Reagents pages incomplete:

• • • Some location pictures not finished or uploaded.

• • • Some newer reagents not entered.

• • Secondary reagents pages not made:

• • • LB, Plates, TAE , NZY+ Broth

• • Protocol part:

• • • No connections from Protocols to reagents.

• • • Ligation protocol missing

• • Lab notebook:

• • • Format uses too many lines to see connections between days.

• • • Latter part missing links

• • • Can table of contents be restricted to date level?

• • Experimental reports:

• • • Lacks discussion/conclusion

• • • Ligation experiment missing

• • • Lattest few gells not marked up and entered.

• • Project overview link broken

• • Background:

• • • GFP missing ,

• • • Red photo sensor to GFP test harness missing.

• • Plan: Only Gas vesicles plan entered.

Individual	Desired time committment
Pat	About 4h/wk Monday afternoon or Tuesday morning
Alisa	About 4h/wk - Thursday afternoon
Jan	About 10h/pw - flexible weekday mornings
Phil	About 18h/pw - Thursday morning, Friday, Saturday or Sunday,