NYMU Taipei/Primer List
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Project Description /
Lab Notes /
Part List /
Primer List
| Name | Sequence | Length | Tm (C) | RE site | Target length | Description |
|---|---|---|---|---|---|---|
| M_INS_A F | GTTTCTTCTCTAGAaaagaggagaaaACTAGATGGGCATTGTAGATCAGTGCTG | 54 bp | 56.85 | XbaI | 119 bp | Potential dimer formation! XbaI+RBS |
| M_INS_A R | GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTAGTTGCAGTAGTTCTCCAGCT | 48 bp | 54.74 | SpeI, PstI | 119 bp | Potential dimer formation! |
| M_INS_B F | GTTTCTTCTCTAGAaaagaggagaaaACTAGATGTTTGTCAAGCAGCACCTTTGT | 55 bp | 60.85 | XbaI | 149 bp | Potential dimer formation! |
| M_INS_B R | GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTAGGACATGGGTGTGTAGAAGA | 48 bp | 55.86 | SpeI, PstI | 149 bp | Potential dimer formation! |
| TAT F | GTTTCTTCTCTAGAaaagaggagaaaACTAGATGAACAATAACGATCTCTTTCAGG | 56 bp | 59.92 | XbaI | bp | XbaI+RBS |
| OmpRBS+TAT F | GTTTCTTCTCTAGAtacattttgaaacatctatagcgataaatgaaacatcttaaaagttttagtatcatattcgtaaagaggagaaaACTAGATGAACAATAACGATCTCTTTCAGG | 108 bp | 59.92 | XbaI | bp | XbaI+RBS |
| H_INS_A F | GTTTCTTCTCTAGAaaagaggagaaaACTAGATGGGCATTGTGGAACAATGCTG | 54 bp | 62.45 | XbaI | 122 bp | Potential dimer formation! XbaI+RBS |
| H_INS_A TAT F | CGCTTGCGCCGCAGTCGCACGGCATTGTGGAACAATGCTG | bp | bp | |||
| TAT H_INS_A R | CAGCATTGTTCCACAATGCCCGCTTGCGCCGC | 32 bp | 59.26 | bp | Potential dimer formation! | |
| H_INS_A R' | GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTAGTTGCAGTAGTTCTCCAGCTG | 49 bp | 57.77 | SpeI, PstI | 122 bp | Potential dimer formation! |
| H_INS_B F | GTTTCTTCTCTAGAaaagaggagaaaACTAGATGTTTGTGAACCAACACCTGTG | 54 bp | 57.98 | XbaI | 149 bp | Potential dimer formation! XbaI+RBS |
| H_INS_B TAT F | CGCTTGCGCCGCAGTCGCACTTTGTGAACCAACACCTGTG | bp | bp | |||
| TAT H_INS_B R | CACAGGTGTTGGTTCACAAACGCTTGCGCCGC | bp | bp | |||
| H_INS_B R | GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTAGGTCTTGGGTGTGTAGAAGA | 48 bp | 55.10 | SpeI, PstI | 149 bp | |
| RcsC F | GTTTCTTCTCTAGAaaagaggagaaaACTAGATGGAAATACCTTGCTTCTTTTCG | 54 bp | 55.92 | XbaI | 1404 bp | XbaI+RBS |
| RcsC R | GTTTCTTCCATATGCAACGACTCTTCCATCTTCAC | 34 bp | 59.63 | NdeI | 1404 bp | BioBrick standard spacer was added to the 5' side of the NdeI cutting site. |
| [http://partsregistry.org/Part:BBa_G00100 VF2] | tgccacctgacgtctaagaa | 20 bp | 59.44 | Forward sequencing primer. | ||
| [http://partsregistry.org/Part:BBa_G00101 VR] | attaccgcctttgagtgagc | 20 bp | 59.34 | Reverse sequencing primer. | ||
| M_IDE TAT F | CGCTTGCGCCGCAGTCGCACCGGAACGGGCTCGTGTGGCT | 40 bp | bp | Potential dimer formation! | ||
| TAT M_IDE R | AGCCACACGAGCCCGTTCCGCGCTTGCGCCGC | bp | bp | |||
| M_IDE R | GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTAGAGTTTTGCAGCCATGAAGT | 48 bp | 54.74 | SpeI, PstI | bp | Potential dimer formation! |
| H_IGFBP3 F | GTTTCTTCTCTAGAaaagaggagaaaACTAGATGCAGCGGGCGCGACCCACGCT | 54 bp | XbaI | bp | Potential dimer formation! XbaI+RBS | |
| H_IGFBP3 R | GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTACTTGCTCTGCATGCTGTAGC | 48 bp | 54.74 | SpeI, PstI | bp | Potential dimer formation! |
| H_IGFBP7 F | GTTTCTTCTCTAGAaaagaggagaaaACTAGATGGAGCGGCCGTCGCTGCGCGC | 54 bp | XbaI | bp | Potential dimer formation! XbaI+RBS | |
| H_IGFBP7 R | GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTATAGCTCGGCACCTTCACCTT | 48 bp | 54.74 | SpeI, PstI | bp | Potential dimer formation! |
- [http://www.mdbio.com.tw/pfu.htm pfu primer]
- [http://partsregistry.org/Help:Contents Biobrick Help]
