User:Lihsiangyen

From 2007.igem.org

Personal schedule

Aug 2007

Aug 6

• Determine the total amount of lab work required
• Find all the available relevent protocol in Dr. Chang's lab
• Write down the schedule and deadline in August
• Divid the big project into several small project

Aug 7

• Allocate each small project to different people
• Set small project deadline
• Start phase 1 project
• Determine a regular meeting time for lab work core member

 Aug 10

Useful information:

• Amp stock conc.: 50mg/ml
• Amp working conc,: 50ug/ml
• Kan stock conc.: 50mg/ml
• Kan working conc.: 20~30ug/ml
• Liquid medium: 5ug/ml
• It is difficult to clone DNA segments longer than about 15,000 bp when plasmid are used as the vector.

Aug 11

• EnvZ information: <a href="http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?val=48994873&db=Nucleotide&from=3532538&to=3533890&view=gbwithparts">DNA sequence</a> and <a href="http://www.pir.uniprot.org/cgi-bin/upEntry?id=P0AEJ4">protein domain prediction</a>
• Basic design:

400px

Aug 15

Useful information:

• Planning
• Planning-New
• <a href="http://openwetware.org/wiki/Endy:M9_media/supplemented">Glucose-free M9 media</a>
• Ribosome binding sites information
• <a href="http://partsregistry.org/partsdb/pgroup.cgi?pgroup=terminator">Transcriptional terminator</a>
• <a href="http://partsregistry.org/Featured_Parts:Cell_Death">Cell death gene as a construction tool</a>
• <a href="http://partsregistry.org/partsdb/pgroup.cgi?pgroup=Plasmid">Available vectors</a>
• <a href="http://partsregistry.org/Part:BBa_V1005">DB3.1</a>
• Available reporter fragment
• <a href="http://partsregistry.org/partsdb/pgroup.cgi?pgroup=Regulatory">Available promotor</a>
• <a href="http://partsregistry.org/Part:BBa_G00100">VF2</a> & <a href="http://partsregistry.org/Part:BBa_G00101">VR</a>
• <a href="http://partsregistry.org/Part_Types:Measurement_Systems">measurement system</a>
• <a href="http://partsregistry.org/cgi/htdocs/Assembly/restriction_enzymes.cgi">Standard assembly</a>

cinR & OmpC dual controlled promotor for IDE, IGFBP3 and IGFBP7

• <a href="http://partsregistry.org/Part:BBa_R0077">Part:BBa_R0077:</a>     17L    iGEM 2007 Parts Kit Plate 1    pSB1A2     231 bp (with RBS)
• <a href="http://partsregistry.org/Part:BBa_C0078">Part:BBa_R0078</a>:     15D    iGEM 2007 Parts Kit Plate 1    pSB1A2     225 bp (without RBS)
• <a href="http://partsregistry.org/Part:BBa_C0077">Part:BBa_C0077</a>:     7G       iGEM 2007 Parts Kit Plate 2    pSB2K3     762 bp
• <a href="http://partsregistry.org/Part:BBa_C0076">Part:BBa_C0076</a>:     7E       iGEM 2007 Parts Kit Plate 2    pSB2K3     702 bp

Aug 20

• 重新設計primer: 5'端3~5個GC, 避免與template annealing。

Aug 24

• Check plasmid時找一個切在insert裡的RE check
• Plasmid有排他性，不一定可以把兩個plasmid同時塞在同一隻細菌裡。
• 解決：E. coli會intake glucose的問題。

Sep 2007

Sep 14

• Putative OmpRBS sequence: tacattttgaaacatctatagcgataaatgaaacatcttaaaagttttagtatcatattcgt
•  pOmpC domain: File:POmpC domain.png