From 2007.igem.org

About Us

Welcome to the wiki for Boston University's iGEM 2007 team!

Our team consists of the following members:

Undergraduates: David Shi, Rahul Ahuja, Christian Ling, Danny Bellin

Faculty Advisor: [http://www.bu.edu/dbin/bme/faculty/?prof=tgardner Dr. Timothy Gardner]

Graduate Student Advisors: Frank Juhn, Stephen Schneider

We work at the the [http://www.gardnerlab.bu.edu Gardner Laboratory].

Our Project Plan

Our project is aimed at increasing current production from Shewanella Oneidensis by directed evolution of global transcription factors

Our plan so far:

1. Mutate global transcription factors
   1. Design primers for amplification
   2. Perform error-prone PCR
2. Transform mutated genes into E. coli
   1. Choose plasmids
   2. Restriction enzyme digestion
   3. Ligation
   4. Transformation into E. coli
3. Conjugate E. coli with Shewanella
4. Screen/select for increased current production due to mutations

Week's Goals

Wednesday 5/30

1. Get all protocols
2. Identify materials/prepare order
3. Design Primers
4. Learn about budget/POs

Thursday 5/31

1. Do primer order
2. Start conjugation practice
3. Confirm restriction enzymes, ligases
4. Order confirmed/needed materials
5. Team Revew Meeting

Friday 6/1

1. Evaluate/continue conjugation, practice electroporation for E. coli
2. Meeting with Tim: Budgets/protocols, Pfizer/fundraising, iGEM registration, beads