Toronto/Lab Notebook
From 2007.igem.org
Oct. 3, 2007
Yusuf
SUMMARY:
- Transformed: J06801 (2005), Q04400 (2005), B0034 (2007), J31005 (2007)
- Minipreped I130033 AB, E0433 AB, S03140 AB, J04500 AB, J31003 AB, N1 AB
- Made o/n of DH5a and DH5a-z1 (from July 16 plate - ask Seema if this is ok)
- Length Check:
| Magnitude | | | | | amount | op | total() | print() | | 1000 | + | 1000 | [1000 USD] | | 1000 | + | 2000 | [2000 USD] | | 10000000000 | + | 10000002000 | [10000002000 USD]| | 3000 | - | 9999999000 | [9999999000 USD]|
TO DO:
- Make 5 Kan plates
- Make competent cells
- Make o/n of J06801 (2005), Q04400 (2005), B0034 (2007), J31005 (2007)
- Ligate N1 + S01003
Oct. 2, 2007
Natalie
SUMMARY:
- Made o/n (2 each) of: I130033 AB, E0433 AB, S03140 AB, J04500 AB, J31003 AB, N1 AB
- NOTE: N1 = J34100 C + S01640 B + B0015
TO DO:
- Miniprep all above parts and length check
- Transform:
- J06801 (2005) - it's in the source box (this is a larger composite part containing J06501)
- B0034 - also in source box, if not, get it from Registry plates
- Q04400 - re-transform using more DNA to see if we get more colonies and transform 2005/2006 version in the source box
- J31005 - chloramphenicol resistance, just in case we need it
- Continue cataloging and organization (Charles: I'll be dropping by to help out with that)
Oct. 1, 2007
Time: 3:45 PM - 8:30 PM
Yusuf, Irina
Summary:
- made 5 KAN plates
- made 2 AMP+KAN plates
- made 300 mL LB
Tranformation of following parts:
- I13033 -> KAN
- E0433 -> KAN
- S03140 -> AMP
- J04500 -> KAN
- J31003 -> AMP
- (J23100C + S01640B)+ B0015 -> KAN
Used 4uL of DNA for all transformations
- tried to do DNA extraction for J06501 but somebody already extracted it (wasn't in the well)
- couldn't find it in the igem dna box as well !!!
TO DO:
- MP o/n for the above parts
