Melbourne/Lab BL Notebook/20071005PCR2

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Revision as of 10:49, 11 October 2007 by Alisa (Talk | contribs)
PCR mix PCR program
5ul 10x buffer\\

2.5ul 10x Enhancer

0.6ul dNTPs (25mM stock)

2.5ul MgSO4 (Supplied in PCR kit)

1.5ul Primer BL_psrfA_fwd (10uM stock)

1.5ul Primer BL_psrfA_rev (10uM stock)

1ul Template (pJS34)

0.4ul Pfx Platinum (Invitrogen)

32.9ul ddH2O

94°C - 5'

94°C - 30"

50°C - 35"

68°C - 1' (goto step 2 x30)

68°C -10'

4°C forever

50ul Total
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