Biobrickificreation of composite Ptet + RFP (product = I716251)
Digest pBca9145-Bca1145 (BglII/BsaI, 1783+970, L) Sub into pBca9145-Bca9205 (BamHI/BsaI/AlwNI, 1049+553+449, L) Product is I716251
No Oligos Needed