From 2007.igem.org

Gene Pho80 CODING SEQUENCE

ATGGAAAGCACATCAGGAGAACGTTCCGAAAATATACATGAGGATCAAGGGATACCAAAAGTAATTCTGCCCGCTGATTTTAATAAATGCTCTAGAACTGACCTAGTGGTGCTCATATCACGAATGTTAGTATCGCTGATAGCAATCAATGAAAATTCAGCAACAAAGAAATCTGATGACCAAATTACTTTAACACGATACCATTCTAAGATTCCTCCAAACATATCAATCTTCAACTATTTCATACGACTGACAAAGTTTTCCTCTTTAGAACATTGTGTGCTTATGACATCACTCTATTATATCGATTTATTGCAAACTGTGTATCCTGATTTTACGCTTAATTCGTTGACTGCCCATAGGTTTTTATTAACAGCCACCACAGTCGCAACAAAAGGCTTATGTGATTCGTTCTCAACAAACGCCCATTATGCAAAAGTTGGAGGAGTACGATGTCACGAATTGAATATACTGGAGAACGATTTTTTAAAGAGAGTAAACTACAGAATCATTCCGCGGGATCATAACATTACGTTATGTAGTATAGAGCAAAAACAGAAAAAGTTTGTCATAGATAAAAACGCATTAGGGTCTCTCGATTTGGATTCTTATTCTTACGTTAATCGTCCAAAAAGTGGATATAATGTTCTAGATAAATACTATCGAAGAATAGTTCAGCTGGTGGGTTCCTTTAACGCTTCACCTGATAAGAGTAGAAAGGTTGATTATGTTCTCCCGCCAAATATTGATATAGTGAGTGAAAGTGGCTCCCAAACTACTCAACTAAAGGGGTCGTCATCACCCAATTCTCACTCTTCACAAAAGCGATATTCTGAGGCAAAGGACGCACATATCTATAACAAGCGATCAAAGCCAGATTAA

XbaI cuts in 2 sites 91 and 648

Pho80cds 882 bp e 293aa

XbaI restriction sequence TCTAGA

1)91 bp position 31-32aa SR S=TCT TCC TCA TCG

R=AGA AGG

2)648 bp position 216-217-218aa VLD V=TT GTC GTA GTG

L=TTA TTG CTT CTA CTC CTG

D=GAT GAC

5'3' Frame 1

Met E S T S G E R S E N I H E D Q G I P K V I L P A D F N K C S R T D L V V L I S R Met L V S L I A I N E N S A T K K S D D Q I T L T R Y H S K I P P N I S I F N Y F I R L T K F S S L E H C V L Met T S L Y Y I D L L Q T V Y P D F T L N S L T A H R F L L T A T T V A T K G L C D S F S T N A H Y A K V G G V R C H E L N I L E N D F L K R V N Y R I I P R D H N I T L C S I E Q K Q K K F V I D K N A L G S L D L D S Y S Y V N R P K S G Y N V L D K Y Y R R I V Q L V G S F N A S P D K S R K V D Y V L P P N I D I V S E S G S Q T T Q L K G S S S P N S H S S Q K R Y S E A K D A H I Y N K R S K P D Stop

Virtual Sequence: SEQUENCE 293 AA; 33227 MW; 72918CAF845AE74C CRC64.

    MESTSGERSE NIHEDQGIPK VILPADFNKC SRTDLVVLIS RMLVSLIAIN ENSATKKSDD
    QITLTRYHSK IPPNISIFNY FIRLTKFSSL EHCVLMTSLY YIDLLQTVYP DFTLNSLTAH
    RFLLTATTVA TKGLCDSFST NAHYAKVGGV RCHELNILEN DFLKRVNYRI IPRDHNITLC
    SIEQKQKKFV IDKNALGSLD LDSYSYVNRP KSGYNVLDKY YRRIVQLVGS FNASPDKSRK
    VDYVLPPNID IVSESGSQTT QLKGSSSPNS HSSQKRYSEA KDAHIYNKRS KPD

PHO80 Primer sequences:

t93c 5'-cgctgattttaataaatgctccagaactgacctagtggtgc-3'

t93c antisense 5'-gcaccactaggtcagttctggagcatttattaaaatcagcg-3'

t648c 5'-atcgtccaaaaagtggatataatgtcctagataaatactatcgaagaatag-3'

t648c antisense 5'-ctattcttcgatagtatttatctaggacattatatccactttttggacgat-3'