BerkiGEM2007-DavidConstructionFile105

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Biobrickificreation of T7 Promoters I716105, I716106, I716107 (Wild Type, Mutant D, Mutant E)

PCR ca1090F and dt008-PT7-wt (62 bp, EcoRI/BamHI, Product = A) PCR ca1090F and dt009-PT7-D (62 bp, EcoRI/BamHI, Product = D) PCR ca1090F and dt010-PT7-E (62 bp, EcoRI/BamHI, Product = E) Sub each separately into pBca9145-Bca1144 (EcoRI/BamHI, 2063+910, L) Product A is pBca9145-I716105-PT7-wt Product D is pBca9145-I716105-PT7-D Product E is pBca9145-I716105-PT7-E

dt008-PT7-wt T7 Promoter (Wild Type) GtttGGATCCaccTCTCCCTATAGTGAGTCGTATTAtacAGATCTcatGAATTCcagaa dt009-PT7-D T7 Promoter (Mutant "D") GtttGGATCCaccTCTCCCTTTAGTGAGTCGTATTAtacAGATCTcatGAATTCcagaa dt010-PT7-E T7 Promoter (Mutant "E") GtttGGATCCaccTGCTTCTATAGTGAGTCGTATTAtacAGATCTcatGAATTCcagaa

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