Tokyo Tech
From 2007.igem.org
1,ProjectConcept & ModelWe made a bacterial society that follows Pareto's principle as an Ant society does.
RequirementsOur requirements to construct this model are two things, bistability and coexistence by cell-cell communication.Genetic circuitWe designed the genetic circuit to satisfy its requirements and developed necessary new parts.
Our constructed parts are Here!!!Our GOALWe indicated our goal of the project by the simulation!! The simulation proved that our genetic circuit have existence of solution.
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2,WorksApproachCombination of Wet & Dry trialsWe have alternated/combined Wet and Dry experiments to achieve our goal 0.Wet : Hybrid promoter
1.Dry : FormulationWe wrote the Formulation 2.Wet : AssayWe decided the parameter of hill coefficients by the assay of AHL & IPTG
3.Dry : Simulationwet実験で求められたパラメータを用いて、集団が怠け者の細胞のみとなった場合に、一度不安定化して、続いて働き者と怠け者になれることをStocastic Simulationで確認した。このシステムの挙動に必要な他のパラメータ範囲が判明した。
Based on stochastic simulation using parameters obtained from our wet experiments, we have confirmed that the whole system becomes unstable when there are only idlers left, and then, they become either of workers and idlers. Also we have determined other parameters necessary for the desired behavior of this system.
4.Wet : AssaySimulationを満たすための他のパラメータが現在のプラスミドで可能であるかを、大腸菌を用いた実験で確認した。
5.Future worksBased on the results from the wet experiments in the step 4, the next dry approach - analysis and simulation - should be performed, which will in turn be testified and confirmed by wet experiments. Continueing these processes, if time permits, would further sophisticate our model.
3,About our team
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