From 2007.igem.org

Our finally vector will be YCplac22,an YC-type (centromeric) shuttle vector.

Vector Information:

Vector type: plasmid

Size (kb): 4.854

Construct size (kb): 4.854

Construction: pUC19

Markers: TRP1, ampR

Features: insert detection: lacZ'

marker(s): TRP1, ampR

promoter: lac

replicon: ARS1, pMB1

MCS: HindIII...EcoRI

centromere: CEN4

Selected features

lac_promoter 142 - 171

M13_pUC_rev_primer 185 - 207

M13_reverse_primer 206 - 224

M13_forward20_primer 306 - 290

M13_pUC_fwd_primer 321 - 299

CEN4 504 - 1356

Orf frame 2 2740 - 2066

TRP1 2740 - 2066

AmpR_promoter 2983 - 3011

Orf frame 2 3053 - 3913

Ampicillin 3053 - 3913

pBR322_origin 4068 - 4687