Tokyo/New AHL check
From 2007.igem.org
Contents |
Purpose:
To determine and compare the activity of the AHL used so far and that of the newly perchased AHL.
Samples:
A4 placQI in pTrc99A (pos. con.)
A4ΔP in pTrc99A (neg. con.)
A4 hybrid promoter in pBR322
Procedure:
The old and the newly delivered AHL was used.
AHL assay Standard protocol
The final concantrations of AHL applied here were 0.01, 0.1, 0.5, 1, 10, 100, and 1000 nM.
