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University of Cambridge

iGEM 2007 wiki

Welcome to the University of Cambridge's iGEM 2007 wiki!

These are the projects we are undertaking this year:

Standardising the Agr signalling system

The Accessory Gene Regulator (Agr) system in S. aureus is an oligopeptide-based quorum sensing mechanism. We aim to convert it into a reusable interbacterial signalling system for communication amongst and between both Gram-positive species (B. subtilis) and Gram-negative bacteria (E. coli), based on controllable secretion and detection of the signalling peptide AIP.

For more information, see Signalling project description.

Bacillus subtilis SynBio Chassis

Most synthetic biology so far has involved Gram-negative bacteria, which are more widely used in molecular biology research; however, the biotechnology industry relies on Gram-positive bacteria. We are trying to make a "chassis" out of Bacillus subtilis, a common Gram-positive bacterium, into which genetic engineers can add BioBrick parts and control circuits.

For more information, see Bacillus subtilis SynBio chassis

PoPS amplifier

We aim to build a standard amplifier which can be used in any synthetic (BioBricked) system, which would take in a standard PoPS input and give a PoPS output of a known amplification factor.

For more details, have a look at the amplifier project.

Some links to explain synthetic biology and the iGEM competition:

[http://en.wikipedia.org/wiki/Synthetic_biology What is synthetic biology?]
[http://www.nature.com/nature/comics/syntheticbiologycomic/index.html Comic of SynBio published in Nature]
[http://www.igem2006.com/story.htm The iGEM story]
Teams taking part in iGEM 2007

Internal links

Groundwork

The project!

Peptide signalling: description, technical notes
B. subtilis chassis: description, technical notes
Amplifier project: description, technical notes
Experimental protocols
Our biobricks
Talks we have given
Useful software

Others

Endgame
Boston, here we come: the trip, preferred flight times
Social events
Brochure, mug and T-shirt design
Wiki help page
Friday afternoons

External links

Our team page on the official iGEM2007 wiki
[http://igem2007.collectivex.com/main/summary Our CollectiveX discussion site]
[http://partsregistry.org The Registry of Standard Biological Parts]
Official iGEM 2007 homepage
[http://www.igem2006.com Offical iGEM 2006 website]
Past Cambridge iGEM wikis: [http://www.ccbi.cam.ac.uk/iGEM2005 2005], [http://www.ccbi.cam.ac.uk/iGEM2006 2006]

Announcements

Stephanie has been looking into taxis to the airport, prices can be found on Taxis or just the cheapest at the trip.
Work in progress: documentation of the signalling project, Bacillus subtilis SynBio chassis and amplifier project description, project description on our page on the official iGEM wiki, (continual) redesigning of wiki layout
Please direct any suggestions you have about the layout to the wiki people!
Record experimental protocols when you use them!
Have you done your visa application?

Contact us!

Meet the brains behind the project!

The mailing list for the whole Cambridge iGEM2007 team is igem2007 [at] ccbi.cam.ac.uk

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