From 2007.igem.org

Overview

Breast cancer, according to the [http://www.who.int World Health Organization (WHO)] [http://www.who.int/mediacentre/factsheets/fs297/en/ (ref)], is the leading cause of cancer deaths in women worldwide. As a significant cause of cancer deaths, breast cancer serves as an appropriate cancer to target in a proof of concept implementation of our general design using the established MCF-7 cell line.

Circuits

The genetic circuits for systems of varying complexity are given below, including a basic system, an AND gate, an AND OR gate.

Basic circuit

Figure 1. image: Basic circuit based on GATA3 mRNA levels only for a MCF-7 cell.

AND circuit

Figure 2. image: AND circuit based on multiple biomarkers for a MCF-7 cell.

AND OR circuit

Figure 3. image: AND OR circuit based on multiple biomarkers for a MCF-7 cell.