USTC/OperatorPosition

From 2007.igem.org

Operator positioning plays a role in determining in vivo level of repression[1].Both operators with strong and weak repression are required to the gates . So we tested the positioning effects systematically.

Figure 1 Left: the influence on the promoter’s activity when a single operator is at a different position upstream of the consensus sequence. Right: the influence on the promoter’s activity when a single operator is at a different position downstream of the consensus sequence.

Figure 2 Assumed influence on solo-repression when an operator is at a different position.

References

1. Elledge, S. J. & Davis, R. W. (1989), 'Position and density effects on repression by stationary and mobile DNA-binding proteins.', Genes Dev 3(2), 185--197.