Wisconsin/Agarose Gel

From 2007.igem.org

< Wisconsin
Revision as of 19:24, 4 July 2007 by Klapoetke (Talk | contribs)

1.2% Agarose Gel preparation

  • 8 wells
  • 600mg Agarose
  • 50mL TBE
  • 1uL 10mg/ml EtBr

Mix agarose and TBE in flask, microwave to make sure agarose is dissolved in TBE. Add EtBr and pour into gel box.


  • 2uL DNA
  • 12uL ddH2O
  • 4uL 5x Dye
  • DNA Ladder

Mix everything in tube before adding to well and ake sure gel box is submerged in TBE before adding DNA.