Alberta/Calender/July
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July
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July 1
July 2
July 3
July 4
Lab Notes:
Transformations of: J61003 (vector containing GFP), J23119 (constitutive promotor), E1010 (RFP), J45100 (methyl salicylate), 0034 (RBS). Transform into XL10 gold cells. Plate on Amp.
-ED, JG, ML, MC, VH, AL, CZ
July 5
July 6
Lab Notes:
Transformation of E1010 did not work, it is in a Kan vector. SMRT.
Set up 4X5mL overnights of pSB1A3, J45100, B0034, J61003.
-ED
July 7
Lab Notes:
Glycerol stocks of overnights from July 6. Stored in -80 freezer. Miniprep from overnights started July 6. Stored in "iGEM" freezer box in -20 freezer.
-JP,JB,AL
July 8
Lab Notes:
Transform E1010 and A0500 into XL10 Gold. Plate on Kan.
Pour Kan and Amp plates. Make TBE and TAE buffer.
-ED, JP, MC, VH, CZ
July 9
Lab Notes:
Retransform E1010 and A0050 into XL10Gold cells using new plates that were poured Sunday.
-ED, VH, JG
July 10
Lab Notes:
Transformations of E1010 and A0050 were not successful. Tomorrow we will try with a new cell line and enriched media.
-ED, MC, AF, CZ
July 11
Lab Notes:
Third attempt at transforming the Kan resistant BioBricks. Transformed E1010, A0500, and pSB2K3 into XL10 Gold, XL10 Gold with enriched media (Magnesium & Glucose), and DH5a cells. Plated 100uL and the pellet remaining after taking the 100uL aliquot on Kan plates.
Restriction digest of J61003 with Xba and Spe. Run on 0.8% agarose gel. Cut out 2290 band, will gel extract tomorrow.
-ED, MC, NG, JG, VH, CZ
July 12
General Notes:
Calender up and running.
Meeting tonight at 7:00 in CSC 2-49.
Received Chlorobium tepidum, meeting with Dr. Jeff Fuller (with Capital Health) to discuss the use of their anaerobic chambers!! -Justin
Lab Notes:
Transformations with DH5a cells worked, but we will need to select for single colonies tonight.
Streak for single colonies of E1010, A0500, J61003.
Purification of gel slice of digested J61003.
-ED, JG, AL, JB, MC, CZ
July 13
General Notes:
Everyone who is coming tonight please be present 7:00pm sharp
Lab Notes:
Started 4X5mL overnights of E1010 and I0050 at 8:30 am. Should be ready for miniprep tonight.
-ED, JB
No growth - made some Kan plates
-MC, JP, JG, CZ
July 14
Schedule:
AL,JP,NK
General Notes:
If you are scheduled during the day please be in the lab at 1230hrs (PM) Don't forget that the Bio-Sci doors lock at 7:00 so if you are trying to get in after 1900hrs call 492-2911
July 15
Schedule:
Jp,JB,AL
July 16
Schedule:
ED,VH,AF
July 17
Schedule:
CY,JG,AF
July 18
Schedule:
CY,MC,NG
July 19
Meeting at 7:00, Room TBA.
Schedule:
ED,MC,VH
July 20
Schedule:
JG,ED,MC
July 21
Schedule:
JP,NK,NG
July 22
July 23
July 24
July 25
July 26
Meeting at 7:00, Room TBA.
July 27
July 28
July 29
July 30
July 31
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