Saint Petersburg\Theoretical study
From 2007.igem.org
The Tri-stable Toggle Switch project was performed by Brown iGEM2006 team, but we need to adapt this to our needs.
Simple Trigger
The main part of system is simple trigger, containing two repressors and to promotors. Each repressor inhibit another repressor synthesis.
Simple trigger
Concentration inhibitors x and y describes the next system equations.
Here the constants D, K, n and r are used to describe the promoter strength and inhibition efficiency.
This system can hold state with high concentration of one inhibitor, and low concentraion of another inhibitor. But we need to have only one stable state, another state must be unstable, and exist only in high copper concentrations.
